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DOI 10.34014/2227-1848-2023-3-14-29

MECHANISMS OF NATURAL MITOCHONDRIAL TRANSFER IN HEALTH AND IN CANCER

O.I. Kit, E.M. Frantsiyants, A.I. Shikhlyarova, I.V. Neskubina

National Medical Research Center for Oncology, Ministry of Health of the Russian Federation,
Rostov-on-Don, Russia

 

This review discusses issues related to mitochondrial dynamics. It also highlights mechanisms allowing these organelles to transcend cell boundaries and transfer between mammalian cells. Mitochondria play a key role in energy generation and cellular physiological processes. These organelles are highly dynamic; they constantly change their morphology, cellular location, and distribution in response to cellular stress.

In recent years, the phenomenon of mitochondrial transfer has attracted significant attention and interest from biologists and medical investigators. Intercellular mitochondrial transfer occurs in a different way, including tunneling nanotubes (TNTs), extracellular vesicles (EVS), and gap junction channels (GJCs). According to research on intercellular mitochondrial transfer in physiological and pathological environments, mitochondrial transfer has great potential for maintaining body homeostasis and regulating pathological processes. Recent evidence also suggests, that cell-free mitochondria release into blood under normal and pathological conditions (stress, trauma). They were found as circulating extracellular mitochondria in blood samples from mica and humans. Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. This paper reviews intercellular spontaneous mitochondrial transfer modes, mechanisms, and the latest methods of AMT/T. Furthermore, potential application value and mechanism of AMT/T in disease treatment (including malignant neoplasms) are also discussed.

Key words: mitochondria, malignant neoplasms, natural mitochondrial transfer, pathological mitochondrial transfer.

 

Conflict of interest. The authors declare no conflict of interest.

Author contributions

Text writing, data analysis and interpretation: Frantsiyants E.M.

Scientific editing: Kit O.I., Shikhlyarova A.I.

Technical editing, reference design: Neskubina I.V.

 

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Received April 03, 2023; accepted June 28, 2023.

 

Information about the authors

Kit Oleg Ivanovich, Doctor of Sciences (Medicine), Professor, Academician of the Russian Academy of Sciences, Director General, National Medical Research Center for Oncology, Ministry of Health of the Russian Federation. 344037, Russia, Rostov-on-Don, 14-ya Liniya St., 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: https://orcid.org/0000-0003-3061-6108

Frantsiyants Elena Mikhaylovna, Doctor of Sciences (Biology), Professor, Deputy Director General for Science, National Medical Research Center for Oncology, Ministry of Health of the Russian Federation. 344037, Russia, Rostov-on-Don, 14-ya Liniya St., 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: http://orcid.org/0000-0003-3618-6890

Shikhlyarova Alla Ivanovna, Doctor of Sciences (Biology), Professor, Senior Researcher, Laboratory for the Study of Malignant Tumor Pathogenesis, National Medical Research Center for Oncology, Ministry of Health of the Russian Federation. 344037, Russia, Rostov-on-Don, 14-ya Liniya St., 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: https://orcid.org/0000-0003-2943-7655

Neskubina Irina Valer'evna, Candidate of Sciences (Biology), National Medical Research Center for Oncology, Ministry of Health of the Russian Federation. 344037, Russia, Rostov-on-Don, 14-ya Liniya St., 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: https://orcid.org/0000-0002-7395-3086

 

For citation

Kit O.I., Frantsiyants E.M., Shikhlyarova A.I., Neskubina I.V. Mekhanizmy estestvennogo perenosa mitokhondriy v norme i pri onkopatologii [Mechanisms of natural mitochondrial transfer in health and in cancer]. Ul'yanovskiy mediko-biologicheskiy zhurnal. 2023; 3: 14–29. DOI: 10.34014/2227-1848-2023-3-14-29 (in Russian).

 

https://orcid.org/0000-0003-3061-6108

УДК 616-092.18;576.311.347

DOI 10.34014/2227-1848-2023-3-14-29

МЕХАНИЗМЫ ЕСТЕСТВЕННОГО ПЕРЕНОСА МИТОХОНДРИЙ В НОРМЕ И ПРИ ОНКОПАТОЛОГИИ

О.И. Кит, Е.М. Франциянц, А.И. Шихлярова, И.В. Нескубина

ФГБУ «Национальный медицинский исследовательский центр онкологии»
Министерства здравоохранения Российской Федерации, г. Ростов-на-Дону, Россия

 

В представленном обзоре обсуждаются вопросы, касающиеся динамической природы митохондрий. Освещаются механизмы, задействованные в способности этих органелл выходить за границы клеток, тем самым позволяя осуществлять их перемещение между клетками млекопитающих. Митохондрии играют ключевую роль в выработке энергии и клеточных физиологических процессах. Эти органеллы очень динамичны, постоянно меняют свою морфологию, расположение в клетке
и распределение в ответ на клеточный стресс.

В последние годы феномен переноса митохондрий привлекает значительное внимание и интерес со стороны биологов и медицинских исследователей. Межклеточный перенос митохондрий происходит различными способами, включая туннельные нанотрубки (TNT), внеклеточные везикулы (EVS) и каналы щелевых соединений (GJC). Исследования межклеточного переноса митохондрий
в физиологических и патологических условиях показали, что митохондриальный перенос обладает большим потенциалом для поддержания гомеостаза организма и регуляции патологических процессов. Недавно стало известно о высвобождении бесклеточных митохондрий в норме и патологических условиях (стресс, травмы) в кровь. Их обнаружили в виде циркулирующих внеклеточных митохондрий в крови мыши и человека. Несколько исследовательских групп разработали методы искусственного переноса / трансплантации здоровых митохондрий (AMT / T) в поврежденные клетки для восстановления клеточной функции. В этой статье рассматриваются способы, механизмы и новейшие методы межклеточного спонтанного митохондриального переноса AMT / T. Кроме того, обсуждается потенциальная ценность и механизм применения AMT / T в лечении заболеваний, в т.ч. и злокачественных новообразований.

Ключевые слова: митохондрии, злокачественные новообразования, естественный перенос митохондрий, перенос митохондрий в условиях патологии.

 

Конфликт интересов. Авторы заявляют об отсутствии конфликта интересов.

Вклад авторов

Написание текста, анализ и интерпретация данных: Франциянц Е.М.

Научное редактирование: Кит О.И., Шихлярова А.И.

Техническое редактирование, оформление библиографии: Нескубина И.В.

 

Литература

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  2. Кит О.И., Франциянц Е.М., Нескубина И.В., Сурикова Е.И., Каплиева И.В., Бандовкина В.А. Влияние варианта развития меланомы В16/F10 на содержание кальция в митохондриях различных органов самок мышей. Исследования и практика в медицине. 2021; 8 (1): 20–29.

  3. Heineman B.D., Liu X., Wu G.Y. Targeted Mitochondrial Delivery to Hepatocytes: A Review. Journal of clinical and translational hepatology. 2022; 10 (2): 321–328.

  4. Porat-Shliom N., Harding O.J., Malec L., Narayan K., Weigert R. Mitochondrial Populations Exhibit Differential Dynamic Responses to Increased Energy Demand during Exocytosis In Vivo. Science. 2019; 11: 440–449.

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Поступила в редакцию 03.04.2023; принята 28.06.2023.

 

Авторский коллектив

Кит Олег Иванович – доктор медицинских наук, профессор, академик РАН, генеральный директор, ФГБУ «Национальный медицинский исследовательский центр онкологии» Минздрава России. 344037, Россия, г. Ростов-на-Дону, ул. 14-я линия, 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: https://orcid.org/0000-0003-3061-6108

Франциянц Елена Михайловна – доктор биологических наук, профессор, заместитель генерального директора по науке, ФГБУ «Национальный медицинский исследовательский центр онкологии» Минздрава России. 344037, Россия, г. Ростов-на-Дону, ул. 14-я линия, 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: http://orcid.org/0000-0003-3618-6890

Шихлярова Алла Ивановна – доктор биологических наук, профессор, старший научный сотрудник лаборатории изучения патогенеза злокачественных опухолей, ФГБУ «Национальный медицинский исследовательский центр онкологии» Минздрава России. 344037, Россия, г. Ростов-на-Дону, ул. 14-я линия, 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: https://orcid.org/0000-0003-2943-7655

Нескубина Ирина Валерьевна – кандидат биологических наук, ФГБУ «Национальный медицинский исследовательский центр онкологии» Минздрава России. 344037, Россия, г. Ростов-на-Дону, ул. 14-я линия, 63; e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра., ORCID ID: https://orcid.org/0000-0002-7395-3086

 

Образец цитирования

Кит О.И., Франциянц Е.М., Шихлярова А.И., Нескубина И.В. Механизмы естественного переноса митохондрий в норме и при онкопатологии. Ульяновский медико-биологический журнал. 2023; 3: 14–29. DOI: 10.34014/2227-1848-2023-3-14-29.